Investigates the worlds of microfluidics and nanotechnologies, and examines the physical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This book contains protocols on the application of microfluidics and the utilization of physical science-related technologies. It is suitable for cellular biologists.
In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In "Micro and Nano Technologies in Bioanalysis: Methods and Protocols", expert researchers from across the globe explore the technology which makes this analysis possible, investigating the worlds of microfluidics and nanotechnologies, and examining physical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This volume contains innovative protocols on the application of microfluidics and the utilization of physical science-related technologies that will prove to be invaluable in the field of molecular biology. Chapters contain cutting edge applications of emerging nanotechnologies, including quantum dots and molecular fluorescence for the imaging and tracking of biomolecules. Composed in the highly successful "Methods in Molecular Biology" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.; Comprehensive and groundbreaking, "Micro and Nano Technologies in Bioanalysis: Methods and Protocols" is a necessary tool for cellular biologists, biochemists, microbiologists, geneticists and medical researchers alike.
Table of Contents Preface Contributors Part I. Applications of microfluidics and nanopores in separation, detection, manipulation, and analysis of biomolecules 1. HPLC-Chip/MS technology in proteomic profiling Martin Vollmer and Tom van de Goor 2. Nanofluidic channels fabrication and manipulation of DNA molecules Kai-Ge Wang, Hanben Niu 3. A single-molecule barcoding system using nanoslits for DNA analysis--Nanocoding Kyubong Jo, Timothy M. Schramm, David C. Schwartz 4. Microfluidic Devices with Photodefinable Pseudo-valves for Protein Separation Z. Hugh Fan 5. Microfluidic chip designed for measuring biomolecules through microbead-based quantum dot fluorescence assay Kwang-Seok Yun, Dohoon Lee, Hak-Sung Kim and Euisik Yoon 6. DNA Focusing Using Microfabricated Electrode Arrays Faisal A. Shaikh and Victor M. Ugaz 7. Solid-state Nanopore for Detecting Individual Biopolymers Jiali Li and Jene A. Golovchenko 8. Inserting and manipulating DNA in a nanopore with optical tweezers U. F. Keyser, J. van der Does, C. Dekker, N. H. Dekker 9. Forming an a-hemolysin nanopore for single molecule analysis Nahid N. Jetha, Matthew Wiggin, and Andre Marziali 10. Nanopore Force Spectroscopy on DNA Duplexes Nahid N. Jetha, Matthew Wiggin, and Andre Marziali Part II. Technologies of physical science and chemistry in detection and analysis of biomolecules 11. Quantitative chemical analysis of single cells Michael L. Heien and Andrew G. Ewing 12. Trapping and Detection of Single Molecules in Water M. Willander, K. Risveden, B. Danielsson and O. Nur 13. ZnO Nanorods as an Intracellular Sensor for pH Measurements M. Willander and Safaa Al-Hilli 14. Analysis of Biomolecules Using Surface Plasmons M. Willander and Safaa Al-Hilli 15. Use of residual dipolar couplings in structural analysis of protein-ligand complexes by solution NMR spectroscopy Nitin U. Jain 16. Raman-assisted X-ray crystallography for the analysis of biomolecules Dominique Bourgeois, Gergely Katona, Eve de Rosny and Philippe Carpentier 17. Methods and Software for Diffuse X-Ray Scattering from Protein Crystals Michael E. Wall 18. Deuterium Labeling for Neutron Structure-Function-Dynamics Analysis Flora Meilleur, Kevin L. Weiss, and Dean A.A. Myles 19. Small-angle Neutron Scattering for Molecular Biology: Basics and Instrumentation William T. Heller, Kenneth C. Littrell 20. Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-molecular Complexes Andrew E. Whitten and Jill Trewhella 21. Protein sequencing with tandem mass spectrometry Assem G. Ziady and Michael Kinter 22. Metabolic Analysis Vladimir V. Tolstikov Part III. Applications of quantum dots and molecular fluorescence in detection, tracking and imaging of biomolecules 23. Multicolor detection of combed DNA molecules using quantum-dots Christophe Escude, Benedicte Geron-Landre, Aurelien Crut, and Pierre Desbiolles 24. Quantum Dot Molecular Beacons for DNA Detection Nathaniel C. Cady 25. Quantum dot hybrid gel blotting: a technique for identifying quantum dot-protein/ protein-protein interactions Tania Q. Vu and Hong Yan Liu 26. In vivo imaging of quantum dots Isabelle Texier, Veronique Josserand 27. Semiconductor Fluorescent Quantum dots: Efficient biolabels in cancer diagnostics Patricia M. A. Farias, Beate S. Santos, Adriana Fontes 28. The Monitoring and Affinity Purification of Proteins Using Dual-Tags with Tetracysteine Motifs Richard J. Giannone, Yie Liu, and Yisong Wang 29. Use of Genomic DNA as Reference in DNA Microarrays Yunfeng Yang 30. Single-molecule Imaging of Fluorescent Proteins Expressed in Living Cells Kayo Hibino, Michio Hiroshima, Masahiro Takahashi, and Yasushi Sako 31. MicroPET, microSPECT, and NIR Fluorescence Imaging of Biomolecules in vivo Zi-Bo Li and Xiaoyuan Chen 32. Ultra-High Resolution Imaging of Biomolecules by Fluorescence Photoactivation Localization Microscopy (FPALM) Samuel T. Hess, Travis J. Gould, Mudalige Gunewardene, Joerg Bewersdorf, and Michael D. Mason Part IV. Nanotechnologies for biomolecular delivery, gene therapy and expression control 33. Real-Time Imaging of Gene Delivery and Expression with DNA Nanoparticle Technologies Wenchao Sun and Assem G. Ziady 34. Nanoparticle-Mediated Gene Delivery Sha Jin, John C. Leach and Kaiming Ye 35. Magnetic nanoparticles for local drug delivery using magnetic implants Rodrigo Fernandez-Pacheco, J. Gabriel Valdivia, and M. Ricardo Ibarra 36. Functionalized magnetic nanoparticles as an in vivo delivery system Shu Taira, Shinji Moritake, Takahiro Hatanaka, Yuko Ichiyanagi, and Mitsutoshi Setou 37. Formulation/preparation of Functionalized Nanoparticles for in vivo Targeted Drug Delivery Frank Gu, Robert Langer, Omid C. Farokhzad 38. Detection of mRNA in single living cells using AFM nanoprobes Hironori Uehara, Atsushi Ikai, and Toshiya Osada 39. Reverse Transfection Using Gold Nanoparticles Shigeru Yamada, Satoshi Fujita, Eiichiro Uchimura, Masato Miyake, and Jun Miyake 40. Custom-designed Molecular Scissors for Site Specific Manipulation of the Plant and Mammalian Genomes Karthikeyan Kandavelou, and Srinivasan Chandrasegaran 41. Determining DNA sequence specificity of natural and artificial transcription factors by cognate site identifier (CSI) analysis Mary S. Ozers, Christopher L. Warren, Aseem Z. Ansari