Details methods for preparing and examining meiotic tissues, cells, and chromosomes by electron microscopy, indirect immunofluorescence, fluorescent in situ hybridization, and live-cell imaging. This title provides fresh protocols for the study of meiotic chromosome dynamics.
The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In "Meiosis", expert researchers explore recent advances in three main areas, including: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. "Meiosis: Volume 2, Cytological Methods", details methods for preparing and examining meiotic tissues, cells, and chromosomes by electron microscopy, indirect immunofluorescence, fluorescent in situ hybridization, and live-cell imaging. The methods in this volume are grouped for convenience by general types of organisms: fungi, plants and small animals (mostly invertebrates), and mammals.; Composed in the highly successful Methods in "Molecular Biology[trademark]" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, "Meiosis: Volume 2, Cytological Methods" is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.
Part I. Cytological Analysis of Meiotic Chromosomes in Fungi Chromosome Spreading and Immunofluorescence Methods in Saccharomyces cerevisiaeBeth Rockmill Analysis of Schizosaccharomyces pombe Meiosis by Nuclear SpreadingJosef Loidl and Alexander Lorenz Assaying Chromosome Pairing by FISH Analysis of Spread Saccharomyces cerevisiae NucleiBeth M. Weiner and Nancy Kleckner Live-Cell Fluorescence Imaging of Meiotic Chromosome Dynamics in Schizosaccharomyces pombeHaruhiko Asakawa and Yasushi Hiraoka Time-Lapse Fluorescence Microscopy of Saccharomyces cerevisiae in MeiosisMichael E. Dresser Real-Time Imaging of Meiotic Chromosomes in Saccharomyces cerevisiaeRomain Koszul, Sei Kameoka, and Beth M. Weiner Observing Meiosis in Filamentous Fungi: Sordaria and NeurosporaDenise Zickler Meiotic Cytogenetics in Coprinus cinereusMiriam E. Zolan and Patricia J. Pukkila Part II. Cytological Analysis of Meiotic Chromosomes in Plants and Small Animals Cytological Analysis of Arabidopsis thaliana Meiotic ChromosomesSusan J. Armstrong, Eugenio Sanchez-Moran, and F. Chris H. Franklin Electron Microscopic Immunogold Localization of Recombination-Related Proteins in Spreads of Synaptonemal Complexes From Tomato MicrosporocytesSteven M. Stack and Lorinda K. Anderson Cytological Analysis of Meiosis in Caenorhabditis elegansCarolyn M. Phillips, Kent L. McDonald, and Abby F. Dernburg Cytological Analysis of Meiosis in Fixed Drosophila OvariesKim S. McKim, Eric F. Joyce, and Janet K. Jang Analysis of Chromosome Dynamics and Chromosomal Proteins in Drosophila SpermatoctyesSharon E. Thomas and Bruce D. McKee Methods for Meiotic Chromosome Preparation, Immunofluorescence, and Fluorescence in situ Hybridization in Daphnia pulexDai Tsuchiya, Brian D. Eads, and Miriam E. Zolan Immunofluorescent Microscopic Study of Meiosis in ZebrafishNazafarin Kochakpour Part III. Cytological and Histological Analysis of Mammalian Germ Cells and Meiotic Chromosomes Staging of Mouse Seminiferous Tubule Cross-SectionsEmad A. Ahmed and Dirk G. de Rooij Isolation and Short-Term Culture of Mouse Spermatocytes for Analysis of MeiosisSophie La Salle, Fengyun Sun, and Mary Ann Handel Isolation and Analyses of Enriched Populations of Male Mouse Germ Cells by Sedimentation Velocity: The Centrifugal ElutriationMarco Barchi, Raffaele Geremia, Roberto Magliozzi, and Enrica Bianchi Electron Microscopy of Mammalian ChromosomesPeter Moens Analyzing Mammalian Female MeiosisMartha Susiarjo, Carmen Rubio, and Patricia Hunt Cytological Analysis of Interference in Mouse MeiosisEsther de Boer, Franck G. P. Lhuissier, and Christa Heyting Analysis of Telomere Dynamics in Mouse SpermatogenesisHarry Scherthan Immunofluorescence Analysis of Human SpermatocytesEvelyn Ko and Renee H. Martin Cytological Techniques to Study Human Female Meiotic ProphaseIgnasi Roig and Montserrat Garcia-Caldes Using RNA FISH to Study Gene Expression During Mammalian MeiosisShantha K. Mahadevaiah, Yael Costa, and James M.A. Turner