Details methods for culturing and manipulating commonly used model organisms and protocols for detecting and quantifying meiotic recombination events through genetic and physical analysis of DNA-DNA or Protein-DNA interactions. This title provides protocols for the study of meiotic chromosome dynamics.
The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In "Meiosis", expert researchers explore recent advances in three main areas, including: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. "Meiosis: Volume 1, Molecular and Genetic Methods" details methods for culturing and manipulating commonly used model organisms and protocols for detecting and quantifying meiotic recombination events through genetic and physical analysis of DNA-DNA or Protein-DNA interactions. Methods are provided for studies in yeasts, plants, and animals, including mouse and human.; Composed in the highly successful "Methods in Molecular Biology[trademark]" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, "Meiosis: Volume 1, Molecular and Genetic Methods" is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.
Part I. Genetic Methods for Studying Meiotic Recombination and Chromosome Dynamics Interaction of Genetic and Environmental Factors in Saccharomyces cerevisiae Meiosis: The Devil is in the DetailsVictoria E. Cotton, Eva R. Hoffmann, Mohammed F. F. Abdullah, and Rhona H. Borts Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain BackgroundsSusan L. Elrod, Sabrina M. Chen, Katja Schwartz, and Elizabeth O. Shuster Modulating and Targeting Meiotic Double-Strand Breaks in Saccharomyces cerevisiaeAlain Nicolas Methods for Analysis of Crossover Interference in Saccharomyces cerevisiaeFranklin W. Stahl and Elizabeth Housworth Measurement of Spatial Proximity and Accessibility of Chromosomal Loci in Saccharomyces cerevisiae Using Cre/loxP Site-Specific RecombinationDoris Lui and Sean M. Burgess Genetic Analysis of Meiotic recombination in Schizosaccharomyces pombeGerald R. Smith Analysis of Meiotic Recombination in Caenorhabditis elegansKenneth J. Hillers and Anne M. Villeneuve Visual Markers for Detecting Gene Conversion Directly in the Gametes of Arabidopsis thalianaLuke E. Berchowitz and Gregory P. Copenhaver Part II. Molecular Analysis of Recombination and Protein-DNA Interactions During Meiosis Gel Electrophoresis Assays for Analyzing DNA Double-Strand Breaks in Saccharomyces cerevisiae at Various Spatial ResolutionsHajime Murakami, Valerie Borde, Alain Nicolas, and Scott Keeney Genome-Wide Mapping of Meiotic DNA Double-Strand Breaks in Saccharomyces cerevisiaeCyril Buhler, Robert Shroff, Michael Lichten Detection of Meiotic DNA Breaks in Mouse Testicular Germ Cells Jian Qin, Jaichandar Subramanian, and Norman Arnheim End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in Saccharomyces cerevisiaeMatthew J. Neale and Scott Keeney Detection of SPO11-Oligonucleotide Complexes from Mouse TestesJing Pan and Scott Keeney Stabilization and Electrophoretic Analysis of Meiotic Recombination Intermediates in Saccharomyces cerevisiaeSteve D. Oh, Lea Jessop, JessicaP. Lao, Thorsten Allers, Michael Lichten, and Neil Hunter Using Schizosaccharomyces pombe Meiosis to Analyze DNA Recombination IntermediatesRandy W. Hyppa and Gerald R. Smith Analysis of Chromatin Structure at Meiotic DSB Sites in yeastsKouji Hirota, Tomoyuki Fukuda, Takatomi Yamada, and Kunihiro Ohta Analysis of Protein-DNA Interactions During Meiosis by Quantitative Chromatin-Immunoprecipitation (qChIP)Marco Antonio Mendoza, Silvia Panizza, and Franz Klein Genome-Wide High-Resolution Chromatin Immunoprecipitation of Meiotic Chromosomal Proteins in Saccharomyces cerevisiaeKazuto Kugou and Kunihiro Ohta Parallel Detection of Crossovers and Non-Crossovers in Mouse Germ CellsFrederic Baudat and Bernard de Massy Analysis of Meiotic Recombination Products from Human SpermLiisa Kauppi, Celia A. May, and Alec J. Jeffreys