Contributes methods to analyze various aspects of the embryogenesis process. Covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, this title addresses features of fetal and placental development as well as of uterine biology.
Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In "Human Embryogenesis: Methods and Protocols", internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.; Authoritative and state-of-the-art, "Human Embryogenesis: Methods and Protocols" provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis.
Part I. Introduction: What is Human Embryogenesis? Human Embryogenesis: Overview Cathy Vaillancourt and Julie Lafond Part II. Studying Embryo Implantation: from Trophoblasts to Embryo Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation. Pavine L.C. Lefevre and Bruce D. Murphy Coculture of Decidua and Trophoblast to Study Proliferation and Invasion Marie Cohen and Paul Bischof Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells Calcium Uptake Frederique Le Bellego, Cathy Vaillancourt and Julie Lafond Endocrinology and Cell Signaling in Human Villous Trophoblast Catherine Mounier, Benoit Barbeau, Cathy Vaillancourt and Julie Lafond Gestation-Induced Uterine Vascular Remodeling Pierre-Andre Scott, Mylene Provencher, Pascale Guerin and Jean St-Louis Part III. Steriodogenesis and Reproductive System Placental and Fetal Steriodogenesis J. Thomas Sanderson Current Methods in Investigating the Development of the Female Reproductive System Patrick J. Devine, Patricia B. Hoyer and Aileen F. Keating A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins Casimir D. Akpov and R.-Marc Pelletier A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis Li Chen, Julie Lafond and R.-Marc Pelletier Part IV. From Embryo to Fetal Development Genetic Dissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow-Cytometry Elodie L. Boulier and Sarah Jenna Immune System: Maturation of Myeloid Cells Jamila Ennaciri and Denis Girard Functional Development of Human Fetal Gastrointestinal Tract Emile Levy, Edgard Delvin, Daniel Menard and Jean-Francois Beaulieu Renal and Cardiac Na+-K+-ATPase and Aconitase in a Rat Model of Fetal Programming Rebecca Gaudet and Michele Brochu Part V. Embryotoxicity, In Vitro Fertilization, Cloning, and Ethics Assessment of Embryotoxicity Using Mouse Embryo Culture Louise M. Winn and Emily W.Y. Tung Fertilization In Vitro Yinzhong Bing and Rodney J. Ouellette Animal Cloning by Somatic Cell Nuclear Transfer Lawrence C. Smith and Jae-Gyu Yoo The Human Embryo: Ethical and Legal Aspects Bartha Maria Knoppers, Sylvie Bordet and Rosario Isasi